|
KMID : 0378019860290020073
|
|
New Medical Journal
1986 Volume.29 No. 2 p.73 ~ p.82
|
|
|
The Ultrastructure of the Skin in the Frog, Rana nigromaculata Hallowell, During Hibernating Phase
|
|
|
|
|
Abstract
|
|
|
This experiment was performed to clarify the morphological differences in the epidermis of the dorsal skin of the frog (Rana nigromaculata Hallowell) during the active ant hibernating phases.
The specimens from the skin were fixed in 2.5% glutaraldehyde (pH 7.2) and % osmium tetroxide solutions, dehydrated with ethanol and acetone series, embedded in Z pon 812, and sectioned with LKB-ultramicrotome. They. were stained with uranyl acetate and lead . - citrate and observed by a Joel 100E electron microscope.
The results were as follows:
1. Amphibian epidermis consisted of 5 or 7 cell layers and was divided into fo r strata; Stratum germinativum, stratum spinosum, stratum granulosum (inner and outer layers), and stratum corneum.
2. Stratum germinativum: In the active phase, rough-endoplasmic reticulum, mit.chondria and ribosomes were well developed in the perinuclear cytoplasm, but in the hibernating phase, rough-endoplasmic reticulums were weakly developed. In the cell adjoining the basement membrane, many microvilli in the intercellular space aggregated densely in the active phase more than the hibernating phase.
3. Stratum spinosum: In the active and hibernating phase, the cells were cu oidal or polygonal in form, and a part of the stratum spinosum was connected with int-1 cellular bridge between cells.
4. Stratum granulosum: In the active phase, it consisted of the inner and oute, layers with a-keratin granules of the same electron density, but in the hibernating phase consisted of the inner layer with a-keratin granules of middle electron density and t e ´outer layer with a-keratin granules of strong electron density. .
5. Stratum corneum: In the active and hibernating phases, it was separated f .m the underlying outer stratum granulosum by the subcorneal space. Semi-cor`nified cells were squamous in form. Microfilaments of weak electron density, a few vacuoles and remnants of cell organelles were seen in the cytoplasm.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|
|